Author: Borislav Stoilov
Stoilov, Borislav, 2023 Crosslinked Collagen-Alginate Hydrogel With PLGA-Loaded Drug For Potential Modulation Of Cell Differentiation In Bone Tissue And Prospective Treatment Of Craniosynostosis, Flinders University, College of Medicine and Public Health
Terms of Use: This electronic version is (or will be) made publicly available by Flinders University in accordance with its open access policy for student theses. Copyright in this thesis remains with the author. You may use this material for uses permitted under the Copyright Act 1968. If you are the owner of any included third party copyright material and/or you believe that any material has been made available without permission of the copyright owner please contact copyright@flinders.edu.au with the details.
Bone tissue plays a crucial role in protecting internal organs, and providing structural support and locomotion of the body. Treatment of hard tissue defects and medical conditions due to physical injuries, aging, metabolic syndromes, infections and not last, genetic disorders, is more often a complex and drawn out process. However, development in the field of nanotechnology, has given the opportunity for potential non-invasive method to be used for treatment of craniosynostosis, as a hard tissue disease.
In that sense, particular drug entities have already been scrutinized to have resulted in inhibiting the mesenchymal progenitor cells in the skull sutures. In this project, the high potent drug molecule GSK-126 was used as a model inhibitor and had been loaded in poly (lactic-co-glycolic acid) nanoparticles. In the scope of the project, the nanoparticles served as a carrier platform within a nanoparticle-hydrogel delivery system. This system had biocompatible, non-toxic, and biodegradable properties due to the composition of the gel vehicle, which included crosslinked collagen and alginate. Firstly, an optimized formulation was reported for preparing the nanoparticles with polyvinylpyrrolidone, as an emulsifying agent, which were then loaded with two placebo molecules, for establishing their morphological characteristics and rate of release– cholecalciferol (vitamin D3) and α-tocopherol (vitamin E). After analyzing the results, the same method was used to prepare GSK-126 loaded nanoparticles in two concentrations. While the morphological and stability features were not satisfactory, the release profiles showed promising results. However, dissolution tests need to be performed on the entire nanoparticle-gel system, and further studies are necessary for optimization and improvement of the drug loaded nanoparticle formulation.
Keywords: Nanoparticles, hydrogel, bone tissue, hard tissue, craniosynostosis, PLGA, GSK-126, collagen, sodium alginate, biodegradable, biocompatible, non-toxic, drug delivery, injectable, model inhibitor
Subject: Medical Biotechnology thesis
Thesis type: Masters
Completed: 2023
School: College of Medicine and Public Health
Supervisor: Prof. Krasimir Vasilev