Expression, Purification and Crystallisation Studies with the M2 Muscarinic and H1 Histamine Receptors.

Author: Amanda Louise Aloia

Aloia, Amanda Louise, 2008 Expression, Purification and Crystallisation Studies with the M2 Muscarinic and H1 Histamine Receptors., Flinders University, School of Biological Sciences

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Abstract

This thesis describes the expression of three human seven transmembrane receptors: the M2 Muscarinic; H1 Histamine and 5HT2A Serotonin receptors, in the baculovirus/insect cell expression system. Purification trials werre conducted on the M2 Muscarinic and H1 Histamine receptors. Preliminary crystallisation attempts were made with the H1 receptor.

Keywords: GPCR,G-protein,seven transmembrane receptor,baculovirus,expression,purification.
Subject: Biological sciences thesis

Thesis type: Doctor of Philosophy
Completed: 2008
School: School of Biological Sciences
Supervisor: Dr Ian Menz