Author: Greta Jane Elizabeth David
David, Greta Jane Elizabeth, 2009 Changes in the innervation of the uterus during pregnancy, Flinders University, School of Medicine
This electronic version is made publicly available by Flinders University in accordance with its open access policy for student theses. Copyright in this thesis remains with the author. This thesis may incorporate third party material which has been used by the author pursuant to Fair Dealing exceptions. If you are the owner of any included third party copyright material and/or you believe that any material has been made available without permission of the copyright owner please contact firstname.lastname@example.org with the details.
Constriction of blood vessels has been linked to reduced blood flow in pre-eclampsia, a hypertensive disorder of pregnancy. This finding suggests that nerves controlling blood vessel diameter may contribute to the pathophysiology of pre-eclampsia. Pregnancy significantly reduces the sympathetic innervation of the uterus and there is some data suggesting that the sensory innervation is also affected. Since sympathetic nerves control blood vessel diameter, a good understanding of the sympathetic innervation of the uterus and how this innervation changes during normal pregnancy is critical for investigating the involvement of sympathetic nerves in the development of pre-eclampsia. Previous histochemical and immunohistochemical studies on uterine innervation have been done on sections. Sections allow only a small sample of the nerves in an organ to be studied whereas whole mounts are capable of providing a picture of all nerves. We therefore developed a method to assess innervation in full-thickness whole mount preparations of non-pregnant uterine horns from rats. Uteri were removed from virgin female rats, bubbled in Ca2+-free Krebs solution, cut open, pinned flat and fixed in 4% formaldehyde. Whole mount preparations of intact, full-thickness uterine horns were stained with an immunoperoxidase method to localize neurochemicals in nerves. Stained whole mounts were dehydrated through graded acetone solutions and propylene oxide. A wire mesh with weight was designed to ensure the whole mounts remained flat during dehydration. After dehydration, the whole mounts were embedded in resin on glass slides under Aclar and polymerised. Uterine whole mounts processed with this method were immunostained throughout their thickness so that all nerves were revealed. Using this whole mount technique, I stained uterine horns from non-pregnant rats in late estrous/early diestrous and rats at day 20 of pregnancy for six neurochemicals that identify different populations of nerves. Tyrosine hydroxylase (TH) and neuropeptide Y (NPY) were considered to be markers for sympathetic nerves; vesicular acetylcholine transporter (VAChT) and nitric oxide synthase (NOS), for parasympathetic nerves; and substance P (SP) and calcitonin gene-related peptide (CGRP), for sensory nerves. The non-pregnant uteri showed significant densities of axons immunoreactive for TH, NPY, SP, CGRP, VAChT and NOS. Dense plexuses of TH- and NPY-containing axons and a few SP-, CGRP-, VAChT- and NOS-containing axons occurred around blood vessels. All six types of axons were present in the muscle.. In non-pregnant rats, the linea uteri, dense bands of longitudinal muscle on the anti-mesometrial side of each uterine horn, contained higher densities of TH, NPY, CGRP and VAChT fibers than the rest of uterine smooth muscle, Uteri from 20-day pregnant rats had very significantly reduced numbers of TH-, NPY-, VaChT- , NOS-, SP- and CGRP-immunoreactive axons. Rare nerves immunoreactive for each of these markers were present on the mesometrial side of the pregnant uterus whereas there were no nerves on the anti-mesometrial side. This thesis describes for the first time the complete innervation of the non-pregnant rat uterus and shows that pregnancy causes sympathetic, parasympathetic and sensory denervation. This information will provide a foundation for future studies on the involvement of sympathetic nerves in the development of pre-eclampsia.
Keywords: whole mount preparation,uterine innervation,pregnancy
Subject: Medicine thesis, Cardiology thesis
Thesis type: Masters
School: School of Medicine
Supervisor: A/Prof. Ida Llewellyn-Smith