Aggregation Induced Emission Bio-probes for Medical Research Application

Author: Margi Patel

Patel, Margi, 2017 Aggregation Induced Emission Bio-probes for Medical Research Application, Flinders University, School of Computer Science, Engineering and Mathematics

Terms of Use: This electronic version is (or will be) made publicly available by Flinders University in accordance with its open access policy for student theses. Copyright in this thesis remains with the author. You may use this material for uses permitted under the Copyright Act 1968. If you are the owner of any included third party copyright material and/or you believe that any material has been made available without permission of the copyright owner please contact with the details.


Non-invasive and long-term method for cell tracking gives critical information on the cell-based assays like to understand structure, development, distribution and behavior of cells. Fluorescent probes used in cell tracking to have cell images. These fluorescent probes are powerful tool in the field of cell based research. In this research study we show that, Water-soluble Aggregation Induced Emission (AIE) fluorogens are used to stain the cells because of its high emission efficiency. Even AIE fluorogens have great cell permeability. In this study BSPOTPE AIEgen is used to stain the human embryonic kidney cells due to its great properties like excellent photo-stability and retention time in cell intracellular compartment comparatively with other commercial dyes. The efficient long time cytoplasm retention time of BSPOTPE gives good choice to its suitability in live cell cytoplasm tracker. The AIE dyes performance opens a new era in fluorescence as bio-probes for monitoring cells and its biological process.

Keywords: cell imaging, fluorescent dyes

Subject: Engineering thesis

Thesis type: Masters
Completed: 2017
School: School of Computer Science, Engineering and Mathematics
Supervisor: Dr. Youhong Tang