Characterisation and disaggregation of murine oviduct to support development of a bioprinted oviduct

Author: Arinze Ezeobi

Ezeobi, Arinze, 2018 Characterisation and disaggregation of murine oviduct to support development of a bioprinted oviduct, Flinders University, College of Medicine and Public Health

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Abstract

The aim of this study was to develop and optimize a method for the disaggregation of murine oviducts that would produce high cell yield and high cell viability. Mouse oviducts were disaggregated using different concentrations of trypsin EDTA, hyaluronidase and collagenase type 1 under different conditions. The use of collagenase type 1 (1%) in combination with mechanical (gentle pipetting) disaggregation produced the highest cell yield and viability when compared to trypsin EDTA and hyaluronidase. A histological study of fixed oviduct tissues that used Periodic Acid Stain (PAS) and hematoxylin and eosin stain (H&E) showed that the different segments of a mouse oviduct contained different types of cells in different proportions.

A novel method was developed to estimate the maximum and minimum number of cells in fixed oviduct tissue. This quantification was used to estimate that the yield of cells from the disaggregated oviducts was 32% of the cells in the parent tissue. Further work is required to confirm the identity of cells in the fixed oviduct tissue, and to identify cells in the mixed cell suspensions obtained from the disaggregated oviducts.

Keywords: Murine oviduct disaggregation

Subject: Biotechnology thesis

Thesis type: Masters
Completed: 2018
School: College of Medicine and Public Health
Supervisor: Assoc. Prof. Fiona Young